Facts-enriched characterization of mobile populations. Morphological information and facts from pictures can incorporate towards the richness of flow cytometry data. Such as, the determine demonstrates an in any other case traditional apoptosis assay working with Annexin V and PI, adding cell imaging to characterize cells in Every single inhabitants to reveal morphologically distinct options. These insights couldn't are actually obtained from multiplex staining by itself.
As samples are obtained over the Attune CytPix Flow Cytometer, the higher-speed brightfield digicam captures and retailers images of detected events at a price approximately six,000 photographs for every second, depending upon the flow fee and graphic sizing. For better versatility, Attune Cytometric Application enables you to regulate the impression capture frequency as wanted and choose a particular gate for image capture, if essential.
(B) RAW 264.7 macrophages will often be annotated inaccurately with regard to particle depend because of their complexity and noncircular borders. The amount of singlets available for analysis will increase after the person trains and employs a far more precise product.
Discover new insights into your experimental info by visualizing populations using put together fluorescence and morphology data. Our program interface wizards enable downsampling, concatenation (merged information) and dimensionality tools which might be developed into your program for an intuitive, streamlined knowledge Examination experience.
"An image just isn't pretty well worth a thousand dots, but it really actually helps you to see photos. It is great to check out photos that correlate with staining. For example, AnnexinV-positive cells which are morphologically distinct than nutritious cells, or CD14+ monocytes which have been more substantial plus much more textured than CD3+/CD4+/CD14- lymphocytes.
Also, it is actually illuminating to determine the amount of doublets slip as a result of standard singlet gates, and excellent to confirm the status of sudden double-favourable situations in my Assessment."
Pair your Attune flow cytometer using an autosampler for enhanced workflow performance, and add robotic integration for maximum throughput.
Regular picture top quality even at substantial throughput Acoustic focusing plus a higher-speed camera Mix to picture these T cells constantly at minimal or large flow costs. Simply regulate focus and digicam options to fulfill experimental demands.
The Attune NxT cytometer would be the dependable workhorse instrument your lab can rely upon for reliable and dependable efficiency. The Attune CytPix product encompasses a superior-speed brightfield digital camera and AI-driven graphic Assessment program, with the latest upgrades which include device Finding out instruments and an intuitive user interface, enabling Improved knowledge precision, precision, and faster insights into sample biology.
End users can view celebration photos in authentic-time to verify sample good quality, optimize protocols, and record the morphology of each mobile population identified for Investigation. Imaging capture also Gains from acoustic concentrating, which helps to place the cells so that a sharp, centered image is attained.
Seem waves help exact cell alignment, resulting in exceptional details high quality and ~10X faster sample flow premiums.
Application note: Automated image Investigation minimizes person-to-user variability in flow cytometry gating strategies
Designed-in dimensionality reduction resources improved resolution into populations. Fluorescently gated populations again-gated onto UMAP plot to verify predicted population resolution in lysed entire human blood. User can watch functions from Every cluster for visual confirmation of mobile phenotypes.
Adaptable payment strategy from Agilent Monetary Options allow you to obtain the essential instrumentation you need for immediate undertaking, or to grow your lab.
Gating on these extended parameters allows you to swiftly and properly establish populations of curiosity and make sure gating technique with little if any guide assessment. The Attune computer software presents use of read more condition-of-the-artwork device Discovering Investigation applications within an expertly created consumer interface, rendering it exceptionally convenient to use.
As the mobile line experienced Beforehand been analyzed within the Attune CytPix Flow Cytometer, the researchers went back again to the images and were in the position to doc the microbial an infection not less than five days earlier.
The Attune CytPix Flow Cytometer allows consumers to visualise samples throughout acquisition, verify the accuracy of gating strategies, and aid outcomes with trustworthy documentation for publication reasons.
What appeared to be a homogeneous population is really extra diverse—an Perception that needs to be regarded when interpreting benefits.